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GeneCorner plasmid details

Last data update: 05 December 2020 04:15 CET

Plasmid name: pCAGGS-FLAG-MDA5-CARD (LMBP 6920)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p6920.gb (View with Genome Compiler)
p6920.txt
p6920.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interferon induced with helicase C domain 1 (IFIH1, MDA5); fragment
FLAG epitope tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS-FLAG-DUBA
Further information: The plasmid was constructed by ligating the PCR amplified caspase recruitment domain (CARD) of human MDA5 cds as a KpnI/XhoI fragment into the KpnI/SalI opened pCAGGS-FLAG-DUBA vector.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726844.1.
The nucleotide sequence of the human MDA5 CARD cds corresponds with the EMBL Nucleotide Sequence Database accession number AF095844.1.
Other name of the plasmid is pCAGGS-flag-MDA5 CARD.
EMBL Accession number: AF095844.1, view at EMBL, GenBank, DDBJ
LT726844.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 11/09/2014
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglII/KpnI, HincII/PvuI and XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Gardam(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-FLAG-MDA5-CARD (LMBP 6920) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Gardam and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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