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GeneCorner plasmid details

Last data update: 21 October 2020 04:26 CEST

Plasmid name: pCAGGS-FLAG-hCASP-1 (LMBP 7477)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p7477.gb (View with Genome Compiler)
p7477.txt
p7477.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human apoptosis-related cysteine peptidase cDNA (caspase-1, CASP1, CASP-1, ICE, P45, IL1BC)
FLAG epitope tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS
Further information: When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726860.1.
The nucleotide sequence of the human CASP1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M87507.1.
Name mentioned in Kersse et al. (2011) is pCAGGS-FLAG-procaspase-1 WT.
Other name of the plasmid is pCAGGS-Flag-hcaspase-1 WT WT.
EMBL Accession number: M87507.1, view at EMBL, GenBank, DDBJ
LT726860.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 07/12/2011
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglII/EcoRI, KpnI, NcoI and SacII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by K. Kersse(1) (2) and Prof. Dr P. Vandenabeele(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Kersse et al., J. Biol. Chem. 286 (2011), 35874-35882 [PMID: 21862576] [DOI: 10.1074/jbc.M111.242321]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-FLAG-hCASP-1 (LMBP 7477) is available at BCCM/GeneCorner. This plasmid was deposited by K. Kersse and Prof. Dr P. Vandenabeele and was published in Kersse et al., 2011.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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