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GeneCorner plasmid details

Last data update: 29 November 2020 04:28 CET

Plasmid name: pCAGGS-Flag-hIKKg (LMBP 6761)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6761.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human NF-κB inhibitor kinase β cDNA (IKBKG, IKK-gamma, NEMO, Fip3p, FIP-3, FIP3 )
FLAG epitope tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS
Further information: The nucleotide sequence of the hIKKg cDNA corresponds with the Genbank accession number NG_009896.1.
Since the nucleotide sequence at the 3' side of the hIKKg cds could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test.
EMBL Accession number: NG_009896.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 02/12/2010
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BanI, HindIII and XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by K. Kersse(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-Flag-hIKKg (LMBP 6761) is available at BCCM/GeneCorner. This plasmid was deposited by K. Kersse and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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