Last data update: 29 November 2020 04:28 CET
Plasmid name: pCAGGS-Flag-mA20-Allmut (LMBP 10395)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse tumor necrosis factor, alpha-induced protein 3 cDNA (Tnfaip3, A20, Tnfip3, GeneID 21929); mutated sequence
FLAG epitope tag; N-terminal
|Promoter:||Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Escherichia coli lac operon promoter
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
|Terminator:||Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Parental clone:||pTwist31_A20Allmut; pCAGGS-FLAGmA20|
|Further information:||The plasmid was constructed by isolating the mutant mouse A20 coding sequence from pTwist31_A20Allmut as an EcoRV/BstXI fragment, and cloning it into the EcoRV/BstXI opened pCAGGS-FLAGmA20 vector.
The mouse A20 coding sequence contains the following mutations: D331A, E332A, N334A, P336A, K337A, E338A, N340A, D343A, D344A, F346A and E347A.
Referring to the sequence analysis results of the Department of Biomedical Molecular Biology (Ghent University, Belgium), the nucleotide sequence of the wild type mouse A20 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U19463.1, except for nucleotide position 2096 of the coding sequence: the T-residue is substituted by a C- residue, resulting in a Leu/Pro replacement.
|EMBL Accession number:||U19463.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||10/08/2017|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||The plasmid was deposited by Prof. Dr G. Van Loo(1)(2). It was constructed by M. Sze(1)(2).
(1) Center for Inflammation Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCAGGS-Flag-mA20-Allmut (LMBP 10395) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr G. Van Loo.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.