Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS-Flag-mA20-K337A-E338A (LMBP 10394)
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Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p10394.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Mouse tumor necrosis factor, alpha-induced protein 3 cDNA (Tnfaip3, A20, Tnfip3, GeneID 21929); mutated sequence FLAG epitope tag; N-terminal |
Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Escherichia coli lac operon promoter Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only |
Ribosome binding site: |
- |
Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pTwist31_A20mut12; pCAGGS-FLAGmA20 |
Further information: | The plasmid was constructed by isolating the mutant mouse A20 coding sequence from pTwist31_A20mut12 as an EcoRV/BstXI fragment, and cloning it into the EcoRV/BstXI opened pCAGGS-FLAGmA20 vector. The mouse A20 coding sequence contains K337A and E338A mutations. Referring to the sequence analysis results of the Department of Biomedical Molecular Biology (Ghent University, Belgium), the nucleotide sequence of the wild type mouse A20 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U19463.1, except for nucleotide position 2096 of the coding sequence: the T-residue is substituted by a C- residue, resulting in a Leu/Pro replacement. Other name of the plasmid is pCAGGS-Flag-mA20-mut12. |
EMBL Accession number: | U19463.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 10/08/2017 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | The plasmid was deposited by Prof. Dr G. Van Loo(1)(2). It was constructed by M. Sze(1)(2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS-Flag-mA20-K337A-E338A (LMBP 10394) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr G. Van Loo. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.