Last data update: 03 December 2020 04:26 CET
Plasmid name: pCAGGS-His-TAT-mABIN-1f-E (LMBP 4232)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human immunodeficiency virus (HIV) trans-activator of transcription cDNA (TAT); protein transduction domain
Mouse A20-binding inhibitor of NF-κB activation 1 cDNA (ABIN-1, TNIP1); minimal NF-κB inhibiting fragment
Histidine tag (His-tag); N-terminal
|Promoter:||Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
|Terminator:||Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Further information:||The plasmid was constructed by inserting an EcoRI PCR amplicon, containing a fusion gene between a fragment of the HIV TAT cDNA and the minimal NF-κB inhibiting mouse ABIN-1 cDNA fragment that consists of nucleotides 1446 to 1919 of the coding sequence, flanked by the N-terminal His-tag and the C-terminal E-tag.
The plasmid is useful for highly efficient expression of the fusion gene under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of the mABIN-1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AJ242778.1.
Although the depositor described the presence of the Kozak consensus sequence ACCATGG at the start of the fusion gene, the sequence file doesn't contain this sequence, as was confirmed by sequence analysis of this region by BCCM/GeneCorner.
Other name of the plasmid is pCAGGS-His-Tat-ABIN(1446-1919)-E.
|EMBL Accession number:||AJ242778.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||30/03/2009|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCAGGS-His-TAT-mABIN-1f-E (LMBP 4232) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.