Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS-TS (LMBP 4167)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4167.gb
(View with Genome Compiler) p4167.txt p4167.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Trypanosoma cruzi trans-sialidase gene (TS); mature sequence |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAGGS |
| Further information: | The plasmid was constructed by inserting the Pfu amplified PCR fragment of the T. cruzi TS gene into the MscI site of pCAGGS. As a result, a unique MscI and an extra NdeI restriction site were created upstream of the start of the coding TS sequence, and a unique BclI and an extra StuI site were created downstream from the coding TS sequence. The TS gene does not contain a C-terminal repeat domain. pCAGGS-TS is useful for highly efficient expression of trans-sialidase under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells. The sequence corresponds with the EMBL Nucleotide Sequence Database accession number AJ276679 (version SV 1). When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727318.1. Other name of the plasmid is pCAGGS-TSj. |
| EMBL Accession number: | AJ276679, view at EMBL, GenBank, DDBJ LT727318.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 25/08/2000 |
| Sequence detail: | Primers used to amplify the TS coding sequence:
Forward: 5' GCCATGGCCAT.ATG.CTG.GCA.CCC.GGA.TCG 3'
***
Reverse: 5' GGCCTAGGCCTGA.TCA.GGC.ACT.CGT.GTC.GCT.GC 3'
+++
***: start codon.
+++: termination codon.
Punctuation indicates reading frame |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII, MscI and StuI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Laroy et al., Protein Expr. Purif. 20 (2000), 389-393 [PMID: 11087678] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS-TS (LMBP 4167) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert and was published in Laroy et al., 2000. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.