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GeneCorner plasmid details

Last data update: 30 October 2020 04:11 CET

Plasmid name: pCAGGS-flag-MAVS (LMBP 6919)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6919.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human mitochondrial antiviral signaling protein (MAVS)
FLAG epitope tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS-FLAG-DUBA
Further information: The plasmid was constructed by ligating the PCR amplified hMAVS cds as a KpnI/XhoI fragment into the KpnI/SalI opened pCAGGS-flag-DUBA vector.
The nucleotide sequence of the hMAVS cds corresponds with the EMBL Nucleotide Sequence Database accession number DQ174270.1.
EMBL Accession number: DQ174270.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 24/02/2011
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Gardam(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-flag-MAVS (LMBP 6919) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Gardam and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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