Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS-mCASP-12 (LMBP 3820)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p3820.gb
(View with Genome Compiler) p3820.txt p3820.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Mouse cysteinyl aspartate specific proteinase 12 cDNA (caspase-12, CASP-12, Casp12) |
Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pCAGGS; pGAD-mCASP-12 |
Further information: | The plasmid was constructed by inserting the mCASP-12 cDNA as a BamHI/MluI fragment from pGAD-mCASP-12 (blunted with Pfu DNA polymerase) into the unique MscI (BalI) site of pCAGGS. pCAGGS-mCASP-12 is useful for highly efficient expression of mCASP-12 under the control of the AG promoter and the hCMV-IE enhancer in various mammalian cells. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727402.1. The nucleotide sequence of the mCASP-12 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number Y13090.1. Other names of the plasmid are pcaggs C34#1(sense) and pcagc34s.. |
EMBL Accession number: | Y13090.1, view at EMBL, GenBank, DDBJ LT727402.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 21/02/2000 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindIII, NcoI, PstI and PvuII. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr P. Vandenabeele(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | Van de Craen et al., FEBS Lett. 403 (1997), 61-69 [PMID: 9038361] |
Related plasmid reference: | Van de Craen et al., Cell Death Differ. 6 (1999), 1117-1124 [PMID: 10578181] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS-mCASP-12 (LMBP 3820) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vandenabeele and was published in Van de Craen et al., 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.