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GeneCorner plasmid details

Last data update: 02 December 2020 04:29 CET

Plasmid name: pCAGGS-mCASP-2 (LMBP 4176)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4176.gb (View with Genome Compiler)
p4176.txt
p4176.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse cysteinyl aspartate specific proteinase 2 cDNA (caspase-2, CASP-2, Casp2, NEDD2, ICH1)
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS; pGAD-mCASP-2
Further information: The plasmid was constructed by inserting the mouse caspase-2 cDNA as a BamHI/MluI fragment of pGAD-mCASP-2 (blunted with Pfu DNA polymerase) into the unique MscI (BalI) site of pCAGGS. The orientation of the inserted fragment is sense relative to the AG promoter.
pCAGGS-mCASP-2 is useful for highly efficient expression of mouse caspase-2 under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727321.1.
The nucleotide sequence of the mCASP-2 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number BC034262.1, except for some 3' UTR nucleotides which correspond to the genomic sequence. These differences are described in the EMBL record.
Other names of the plasmid are pcaggs F72#8 (sense) and pcagf72S.
EMBL Accession number: BC034262.1, view at EMBL, GenBank, DDBJ
LT727321.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 22/06/2000
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Vandenabeele(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Van de Craen et al., FEBS Lett. 403 (1997), 61-69 [PMID: 9038361]
Related plasmid reference: Van de Craen et al., Cell Death Differ. 6 (1999), 1117-1124 [PMID: 10578181]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-mCASP-2 (LMBP 4176) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vandenabeele and was published in Van de Craen et al., 1997.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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