Last data update: 25 October 2020 04:11 CET
Plasmid name: pCAGGS-mCASP-3a (LMBP 3822)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Mouse caspase 3 cDNA (Casp3, AC-3, Apopain, Caspase-3, CC3, CPP32, mldy, Yama, GeneID 12367)
|Promoter:||Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
|Terminator:||Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Parental clone:||pCAGGS; pGAD-mCASP-3|
|Further information:||The plasmid was constructed by inserting the mouse caspase-3 cDNA as a SwaI/SmaI fragment of pGAD-mCASP-3 into the unique MscI (BalI) site of pCAGGS. The orientation of the inserted fragment is antisense relative to the AG promoter.
The AG promoter sequence consists of the chicken β-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit β-globin fragment, consisting of a 3' part of the second intron (inclusive a branch point which is required for normal splicing reactions) and a 5' part of the third exon.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727404.1.
Other name of the plasmid is pCAGGS D41#1(antisense).
|EMBL Accession number:||LT727404.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||29/05/2000|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: AccI/BalI, BamHI, EcoRI, MspA1I, NcoI and XbaI.|
|History of deposit:||This plasmid was deposited by Dr M. Van de Craen(1)(2) and Prof. Dr P. Vandenabeele(1)(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Related plasmid reference:||Van de Craen et al., FEBS Lett. 403 (1997), 61-69 [PMID: 9038361]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCAGGS-mCASP-3a (LMBP 3822) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Van de Craen and Prof. Dr P. Vandenabeele.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.