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GeneCorner plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pCAGGS-mMyD88 (LMBP 5643)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse myeloid differentiation primary response gene 88 cDNA (Myd88, GeneID 17874); complete CDS, Myd88 long (Myd88l)
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS-E-mMyD88
Further information: The mouse myeloid differentiation primary response gene 88 cDNA (Myd88), encoding an adaptor protein involved in TLR and IL-1R induced activation of NF-κB, is expressed under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.
The plasmid is derived from pCAGGS-E-mMyD88; the EcoRI/NotI E-tag was removed, the restriction sites were blunted and religated.
The nucleotide sequence of the mouse Myd88 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U84409.1.
EMBL Accession number: U84409.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 04/07/2007
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Janssens(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Janssens et al., Curr. Biol. 12 (2002), 467-471 [PMID: 11909531]
Related plasmid reference: Janssens et al., Trends Biochem. Sci. 27 (2002), 474-482 [PMID: 12217523]
Janssens et al., FEBS Lett. 548 (2003), 103-107 [PMID: 12885415]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-mMyD88 (LMBP 5643) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Janssens and Prof. Dr R. Beyaert and was published in Janssens et al., 2002.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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