Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGShMnSOD (LMBP 3451)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3451.gb
(View with Genome Compiler) p3451.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human manganese superoxide dismutase cDNA (hMnSOD) |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) Polyoma virus origin |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAGGSPy; pSP-MS84 |
| Further information: | The plasmid was constructed by inserting the human manganese superoxide dismutase cDNA (hMnSOD) as an EcoRI fragment from pSP-MS84 (Y. Beck) into the EcoRI digested pCAGGSPy vector. pCAGGShMnSOD is useful for highly efficient expression of human MnSOD under the control of the chicken β-actin/rabbit β-globin hybrid promoter (AG) and the human CMV-IE enhancer in various mammalian cells. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727340.1. The nucleotide sequence of the human MnSOD cDNA corresponds with the EMBL Nucleotide Sequence Database accession number Y00472.1, with minor corrections in the 5' and 3' UTR after sequence analysis at the Department of Biomedical Molecular Biology (Ghent University, Belgium). Other name of the plasmid is pCAGGS-huMnSOD. |
| EMBL Accession number: | Y00472.1, view at EMBL, GenBank, DDBJ LT727340.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 31/05/2005 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BamHI/HindIII, EcoRI, HindIII, NcoI/PvuI, RsaI and XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1)(2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Beck et al., Nucleic Acids Res. 15 (1987), 9076 [PMID: 3684581] Beck et al., Biotechnology 6 (1988), 930-935 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGShMnSOD (LMBP 3451) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.