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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pCAGGSpPHGPx (LMBP 4431)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4431.gb (View with Genome Compiler)
p4431.txt
p4431.pdf
Sequence
analysis results
Genecorner:

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Cloned DNA: Pig phospholipid hydroperoxide glutathion peroxidase cDNA (PHGPx, GPx4)
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS; pcDNA3pPHGPx
Further information: The plasmid was constructed by inserting the pig PHGPx cDNA as a KpnI (blunted with T4 DNA polymerase)/EcoRV fragment from pcDNA3pPHGPx into the MscI site of pCAGGS.
pCAGGSpPHGPx is useful for highly efficient expression of pig cytosolic PHGPx under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727207.1.
The borders of the inserted fragment have been sequenced at the Department for Molecular Biomedical Research (VIB, Belgium).
The nucleotide sequence of the pig PHGPx cDNA corresponds with the EMBL Nucleotide Sequence Database accession number X76009.1.
Name mentioned in Rubio et al. (2012) is pCAGGS_p-cGPX4.
Other name of the plasmid is pCAGGS/pPHGPx.
EMBL Accession number: X76009.1, view at EMBL, GenBank, DDBJ
LT727207.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/03/2006
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: NcoI, PvuII, SphI and XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr J. Grooten(1) (2). It was constructed by V. Goossens(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Heirman et al., Free Radic. Biol. Med. 40 (2006), 285-294 [PMID: 16413410]
Related plasmid reference: Brigelius-Flohé et al., J. Biol. Chem. 269 (1994), 7342-7348 [PMID: 8125951]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGSpPHGPx (LMBP 4431) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J. Grooten and was published in Heirman et al., 2006.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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