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GeneCorner plasmid details

Last data update: 30 October 2020 04:11 CET

Plasmid name: pCAXL-FRB-Mx1 (LMBP 9755)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9755.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse MX dynamin-like GTPase 1 cDNA (Mx1, Mx-1, GeneID 17857)
Homo sapiens mechanistic target of rapamycin kinase (MTOR, FRAP1, FRAP2, RAFT1, RAPT1, GeneID 2475); artificial FRB domain
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAXL-Mx1
Further information: The plasmid was constructed by cloning the artificial heterodimerization domain FRB into the pCAXL-Mx1, at the N-terminus of the mouse Mx1 coding sequence.
The mouse Mx1 cDNA originates from BALB.A2G mice.
The nucleotide sequence of the parental pCAXL vector corresponds with the EMBL Nucleotide Sequence Database accession number LT726945.1.
Other name of the plasmid is pCAXL-FRBMx1_A2G.
EMBL Accession number: LT726945.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 05/11/2019
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr X. Saelens(1)(2)(3). It was constructed by Dr J. Verhelst(1)(2)(3).
(1) Department of Medical Protein Research, VIB, Ghent, Belgium
(2) Inflammation Research Center, VIB, Ghent, Belgium
(3) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5αT1R
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAXL-FRB-Mx1 (LMBP 9755) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr X. Saelens.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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