Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAXL-FRB-Mx1 (LMBP 9755)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9755.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse MX dynamin-like GTPase 1 cDNA (Mx1, Mx-1, GeneID 17857) Homo sapiens mechanistic target of rapamycin kinase (MTOR, FRAP1, FRAP2, RAFT1, RAPT1, GeneID 2475); artificial FRB domain |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAXL-Mx1 |
| Further information: | The plasmid was constructed by cloning the artificial heterodimerization domain FRB into the pCAXL-Mx1, at the N-terminus of the mouse Mx1 coding sequence. The mouse Mx1 cDNA originates from BALB.A2G mice. The nucleotide sequence of the parental pCAXL vector corresponds with the EMBL Nucleotide Sequence Database accession number LT726945.1. Other name of the plasmid is pCAXL-FRBMx1_A2G. |
| EMBL Accession number: | LT726945.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/11/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr X. Saelens(1)(2)(3). It was constructed by Dr J. Verhelst(1)(2)(3). (1) Department of Medical Protein Research, VIB, Ghent, Belgium (2) Inflammation Research Center, VIB, Ghent, Belgium (3) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAXL-FRB-Mx1 (LMBP 9755) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr X. Saelens. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.