GREAT AT SMALL THINGS

0

GeneCorner plasmid details

Last data update: 05 December 2020 04:15 CET

Plasmid name: pCAXL-Mx1-FRB (LMBP 9647)

Add to cart

New search

Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9647.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse MX dynamin-like GTPase 1 cDNA (Mx1, Mx-1, GeneID 17857); mutated sequence
Homo sapiens mechanistic target of rapamycin kinase (MTOR, FRAP1, FRAP2, RAFT1, RAPT1, GeneID 2475); artificial FRB domain
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAXL-Mx1
Further information: The plasmid was constructed by cloning an artificial heterodimerization domain FRB at the 3' end of the mouse Mx1 coding sequence in pCAXL-Mx1.
This plasmid encodes for the Mus musculus A2G Mx1 protein with a C-terminal artificial heterodimerization domain FRB.
The nucleotide sequence of the pCAXL vector corresponds with the EMBL Nucleotide Sequence Database accession number LT726945.1.
Other name of the plasmid is pCAXL-Mx1FRB_A2G.
EMBL Accession number: LT726945.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 06/07/2018
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr X. Saelens(1) (2). It was constructed by Dr J. Verhelst(1)(2).
(1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Verhelst et al., J. Virol. 89 (2015), 10879-10890 [PMID: 26292322] [DOI: 10.1128/JVI.01744-15]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAXL-Mx1-FRB (LMBP 9647) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr X. Saelens .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search