Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAXL-Mx1T69A (LMBP 7970)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7970.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse MX dynamin-like GTPase 1 cDNA (Mx1, Mx-1, GeneID 17857); mutated sequence |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAXL-Mx1 |
| Further information: | The plasmid was constructed by introducing a T69A mutation into the mouse Mx1 cDNA of pCAXL-Mx1 via site directed mutagenesis. The plasmid contains an inactive mutant of BALB.A2G mouse Mx1. The nucleotide sequence of the wild type mouse Mx1 cDNA corresponds with the Genbank accession number NM_010846.1. Other name of the plasmid is pCAXL-Mx1T69A_A2G. |
| EMBL Accession number: | NM_010846.1, view at GenBank |
| Latest sequence update: | 11/06/2015 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Verhelst(1) (2) and Prof. Dr X. Saelens(1) (2). (1) Department of Medical Protein Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Verhelst et al., J. Virol. 86 (2012), 13445-13455 [PMID: 23015724] [DOI: 10.1128/JVI.01682-12] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAXL-Mx1T69A (LMBP 7970) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Verhelst and Prof. Dr X. Saelens and was published in Verhelst et al., 2012. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.