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GeneCorner plasmid details

Last data update: 24 October 2020 04:12 CEST

Plasmid name: pCAXL-Mx1T69A (LMBP 7970)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p7970.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse MX dynamin-like GTPase 1 cDNA (Mx1, Mx-1, GeneID 17857); mutated sequence
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAXL-Mx1
Further information: The plasmid was constructed by introducing a T69A mutation into the mouse Mx1 cDNA of pCAXL-Mx1 via site directed mutagenesis.
The plasmid contains an inactive mutant of BALB.A2G mouse Mx1.
The nucleotide sequence of the wild type mouse Mx1 cDNA corresponds with the Genbank accession number NM_010846.1.
Other name of the plasmid is pCAXL-Mx1T69A_A2G.
EMBL Accession number: NM_010846.1, view at GenBank
Latest sequence update: 11/06/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Verhelst(1) (2) and Prof. Dr X. Saelens(1) (2).
(1) Department of Medical Protein Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Verhelst et al., J. Virol. 86 (2012), 13445-13455 [PMID: 23015724] [DOI: 10.1128/JVI.01682-12]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAXL-Mx1T69A (LMBP 7970) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Verhelst and Prof. Dr X. Saelens and was published in Verhelst et al., 2012.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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