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GeneCorner plasmid details

Last data update: 02 December 2020 04:29 CET

Plasmid name: pCAXL-NB5.D9 escape (LMBP 9323)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9323.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Influenza A virus NIBRG-14 (H5N1) neuraminidase cDNA; mutant
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAXL
Further information: The plasmid was constructed by cloning the NB5 escape mutant D9 of the Influenza A virus NIBRG-14 neuraminidase cDNA into the pCAXL vector.
The NB5 escape mutant D9 of neuraminidase CDS contains a single nucleotide substitution, resulting in a Ile437Thr mutation.
The mutated neuraminidase CDS is cloned in antisense direction compared to the AG promoter of pCAXL.
The influenza reference virus NIBRG-14 is a reassortant prepared by reverse genetics from A/Vietnam/1194/2004 (H5N1) virus (in which the polybasic HA cleavage site has been excised) and A/PR/8/34(H1N1) virus.
EMBL Accession number: KJ867558, view at EMBL, GenBank, DDBJ
Latest sequence update: 29/07/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by S. Van den Hoecke(1) (2) and Prof. Dr X. Saelens(1) (2).
(1) Inflammation Research Center, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Cardoso et al., J. Virol. 88 (2014), 8278-8296 [PMID: 24829341]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAXL-NB5.D9 escape (LMBP 9323) is available at BCCM/GeneCorner. This plasmid was deposited by S. Van den Hoecke and Prof. Dr X. Saelens .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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