Last data update: 23 October 2020 04:27 CEST
Plasmid name: pCAXL-SMx1-GTPaseA2G (LMBP 9656)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse hybrid MX dynamin-like GTPase 1 cDNA (Mx1, Mx-1)
|Promoter:||Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
|Terminator:||Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Further information:||This plasmid is useful for highly efficient expression of hybrid mouse Mx1 under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.
This vector contains the Mus spretus Mx1 in which the GTPase domain is replaced by the GTPase domain of Mus musculus A2G Mx1.
The AG promoter sequence consists of the chicken β-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit β-globin fragment, consisting of a 3' part of the second intron (inclusive a branch point which is required for normal splicing reactions) and a 5' part of the third exon.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
Other name of the plasmid is pCAXL-SMx1_GTPaseA2G.
|EMBL Accession number:||-|
|Latest sequence update:||11/02/2016|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr X. Saelens(1)(2)(3).
(1) Department of Medical Protein Research, VIB, Ghent, Belgium
(2) Inflammation Research Center, VIB, Ghent, Belgium
(3) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Verhelst et al., J. Virol. 89 (2015), 10879-10890 [PMID: 26292322] [DOI: 10.1128/JVI.01744-15]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCAXL-SMx1-GTPaseA2G (LMBP 9656) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr X. Saelens and was published in Verhelst et al., 2015.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.