Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAXL-SMx1-T69A (LMBP 9646)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Mouse MX dynamin-like GTPase 1 cDNA (Mx1, Mx-1); mutated sequence |
Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pCAXL-SMx1 |
Further information: | The plasmid was constructed by introducing a T69A mutation into the Mus spretus Mx1 coding sequence of pCAXL-SMx1. This plasmid encodes for an inactive mutant of the Mus spretus Mx1 protein. The nucleotide sequence of the pCAXL vector corresponds with the EMBL Nucleotide Sequence Database accession number LT726945.1. Other name of the plasmid is pCAXL_SMx1_T69A. |
EMBL Accession number: | LT726945.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 06/07/2018 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr X. Saelens(1) (2). It was constructed by Dr J. Verhelst(1)(2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Related plasmid reference: | Verhelst et al., J. Virol. 89 (2015), 10879-10890 [PMID: 26292322] [DOI: 10.1128/JVI.01744-15] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAXL-SMx1-T69A (LMBP 9646) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr X. Saelens . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.