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GeneCorner plasmid details

Last data update: 29 November 2020 04:28 CET

Plasmid name: pCAXL-SMx1-T69A (LMBP 9646)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse MX dynamin-like GTPase 1 cDNA (Mx1, Mx-1); mutated sequence
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAXL-SMx1
Further information: The plasmid was constructed by introducing a T69A mutation into the Mus spretus Mx1 coding sequence of pCAXL-SMx1.
This plasmid encodes for an inactive mutant of the Mus spretus Mx1 protein.
The nucleotide sequence of the pCAXL vector corresponds with the EMBL Nucleotide Sequence Database accession number LT726945.1.
Other name of the plasmid is pCAXL_SMx1_T69A.
EMBL Accession number: LT726945.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 06/07/2018
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr X. Saelens(1) (2). It was constructed by Dr J. Verhelst(1)(2).
(1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Verhelst et al., J. Virol. 89 (2015), 10879-10890 [PMID: 26292322] [DOI: 10.1128/JVI.01744-15]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAXL-SMx1-T69A (LMBP 9646) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr X. Saelens .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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