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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: pCD-A7M8K (LMBP 5537)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5537.gb (View with Genome Compiler)
p5537.txt
p5537.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human apoptosis inhibitor 2 cDNA (API2, BIRC3); N-terminal fragment (exon 1 --> 7) with Kozak consensus sequence
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1); C-terminal fragment (exon 8 --> end)
Myc epitope; C-terminal
Histidine tag (His-tag); C-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pcDNA3.1/myc-HisA
Further information: The plasmid contains the N-terminal fragment of the hAPI2 coding sequence (exon 1 --> exon 7) fused to the C-terminal fragment of the hMLT coding sequence (exon 8 --> end) and to the C-terminal Myc epitope and histidine tag.
The plasmid is designed for high-level expression of the fusion protein under control of the strong hCMV-IE promoter and enhancer in mammalian cells.
The parental clone of this vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726921.1.
The nucleotide sequence of the wt hAPI2 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number BC037420.1.
The nucleotide sequence of the wt hMLT cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2.
Other name of the plasmid is pCD-API2-MLT.
EMBL Accession number: BC037420.1, view at EMBL, GenBank, DDBJ
AF130356.2, view at EMBL, GenBank, DDBJ
LT726921.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/11/2007
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: Alw44I/HindIII, ApaI/BamHI, NcoI, NdeI, PauI/PstI and PdmI/SalI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. Baens(1).
(1) Department of Human Genetics, KULeuven, Belgium
Plasmid reference: -
Related plasmid reference: Noels et al., J. Biol. Chem. 282 (2007), 10180-10189 [PMID: 17287209]
Coornaert et al., Nat. Immunol. 9 (2008), 263-271 [PMID: 18223652] [DOI: 10.1038/ni1561]
Staal et al., EMBO J. 30 (2011), 1742-1752 [PMID: 21448133] [DOI: 10.1038/emboj.2011.85]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCD-A7M8K (LMBP 5537) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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