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GeneCorner plasmid details

Last data update: 29 October 2020 04:15 CET

Plasmid name: pCD-F-ATPIP-YFP (LMBP 6525)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human ATP synthase inhibitory factor subunit 1 (ATP5IF1, ATPIP, ATPIF1, GeneID 93974)
Aequorea victoria green fluorescent protein DNA (GFP); enhanced yellow-green fluorescent variant (EYFP), C-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCD-F-ATPIP; pdEYFP
Further information: The plasmid was constructed by fusing the ATPIP and YFP genes via fusion PCR and cloning the resulting fragment into the pCD-F vector.
EMBL Accession number: -
Latest sequence update: 03/12/2009
Sequence detail:
Primers used in the fusion PCR:

KpnI-ATPIP-F: atggtacca.ATG.GCA.GTG.ACG.GCG.TT 
                KpnI
ATPIP-xFP-F: atcaaaatgctaaaacatgatgat.ATG.GTG.AGC.AAG.GGC.GAG.G 

ApaI-xFP-R: aagGGGCCCCTTGTACAGCTCGTCCATGC 
               ApaI
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCD-F-ATPIP-YFP (LMBP 6525) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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