Last data update: 24 January 2024 16:39 CET
Plasmid name: pCD-F-Bcl10 (LMBP 5535)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5535.gb
(View with Genome Compiler) p5535.txt p5535.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human BCL10, immune signaling adaptor cDNA (BCL10, c-E10, CARMEN, CIPER, CLAP, mE10, GeneID 8915) FLAG epitope tag; N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3.1-Flag |
| Further information: | The plasmid was constructed by inserting a 719 bp BamHI-ApaI fragment, containing the human BCL10 coding sequence, between the BamHI and ApaI site of pcDNA3.1-Flag. The protein encoded by the wild type BCL10 gene contains a caspase recruitment domain (CARD), induces apoptosis and activates NF-κB. The L41Q mutation would disrupt dimerisation of BCL10 and prevent NF-κB activation of the MLT-BCL10 complex. The parental clone of this vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726919.1. The nucleotide sequence of the human BCL10 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF082283.1. Other name of the plasmid is pCD-Bcl10. |
| EMBL Accession number: | AF082283.1, view at EMBL, GenBank, DDBJ LT726919.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 28/11/2007 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: Alw44I/XbaI, ApaI/BamHI, AvaI/XmnI, BglII/PvuII, HincII/KpnI and NdeI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr M. Baens(1). (1) Department of Human Genetics, KULeuven, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Afonina et al., EMBO Rep. 17 (2016), 914-927 [PMID: 27113748] [DOI: 10.15252/embr.201642109] Elton et al., FEBS J. 283 (2016), 403-412 [PMID: 26573773] [DOI: 10.1111/febs.13597] Staal et al., EMBO J. 30 (2011), 1742-1752 [PMID: 21448133] [DOI: 10.1038/emboj.2011.85] Noels et al., J. Biol. Chem. 282 (2007), 10180-10189 [PMID: 17287209] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCD-F-Bcl10 (LMBP 5535) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.