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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pCD-F-Bcl10 (LMBP 5535)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5535.gb (View with Genome Compiler)
p5535.txt
p5535.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human BCL10, immune signaling adaptor cDNA (BCL10, c-E10, CARMEN, CIPER, CLAP, mE10, GeneID 8915)
FLAG epitope tag; N-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pcDNA3.1-Flag
Further information: The plasmid was constructed by inserting a 719 bp BamHI-ApaI fragment, containing the human BCL10 coding sequence, between the BamHI and ApaI site of pcDNA3.1-Flag.
The protein encoded by the wild type BCL10 gene contains a caspase recruitment domain (CARD), induces apoptosis and activates NF-κB. The L41Q mutation would disrupt dimerisation of BCL10 and prevent NF-κB activation of the MLT-BCL10 complex.
The parental clone of this vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726919.1.
The nucleotide sequence of the human BCL10 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF082283.1.
Other name of the plasmid is pCD-Bcl10.
EMBL Accession number: AF082283.1, view at EMBL, GenBank, DDBJ
LT726919.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/11/2007
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: Alw44I/XbaI, ApaI/BamHI, AvaI/XmnI, BglII/PvuII, HincII/KpnI and NdeI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. Baens(1).
(1) Department of Human Genetics, KULeuven, Belgium
Plasmid reference: -
Related plasmid reference: Noels et al., J. Biol. Chem. 282 (2007), 10180-10189 [PMID: 17287209]
Elton et al., FEBS J. 283 (2016), 403-412 [PMID: 26573773] [DOI: 10.1111/febs.13597]
Staal et al., EMBO J. 30 (2011), 1742-1752 [PMID: 21448133] [DOI: 10.1038/emboj.2011.85]
Afonina et al., EMBO Rep. 17 (2016), 914-927 [PMID: 27113748] [DOI: 10.15252/embr.201642109]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCD-F-Bcl10 (LMBP 5535) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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