Last data update: 22 October 2020 04:25 CEST
Plasmid name: pCD-F-DrMALT1-C444A (LMBP 6462)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Danio rerio MALT lymphoma associated translocation cDNA (PCASP1, MALT1); mutated sequence
FLAG epitope tag; N-terminal
|Promoter:||Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
|Terminator:||Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)
Neomycin (neo; G418)
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Further information:||The plasmid was constructed by introducing a C444A mutation in the zebrafish MALT1 coding sequence of pCD-F-DrMALT1, creating a catalytic cystein mutant.
The nucleotide sequence of the zebrafish MALT1 coding sequence corresponds with the Genbank accession number NM_152976.1.
|EMBL Accession number:||NM_152976.1, view at GenBank|
|Latest sequence update:||01/10/2009|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr M. Baens(1).
(1) Department of Human Genetics, KULeuven, Belgium
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCD-F-DrMALT1-C444A (LMBP 6462) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.