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GeneCorner plasmid details

Last data update: 24 October 2020 04:12 CEST

Plasmid name: pCD-F-EIF4B (LMBP 6359)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pcd-f-eif4b.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human eukaryotic translation initiation factor 4B cDNA (EIF4B)
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCD-F-MALT1
Further information: The human EIF4B cds was PCR amplified from Jurkat cDNA and cloned as a KpnI/ApaI fragment into the pCD-F-MALT1 vector, replacing the human MALT1 cds.
EMBL Accession number: -
Latest sequence update: 09/10/2013
Sequence detail:
Primers used to amplify the human EIF4B cds:

Forward: 5' ATGGTACCA.ATG.GCG.GCC.TCA.GCA.AAA
              KpnI    ***

Reverse: 5' TAAGGGCCC.CTA.TTC.GGC.ATA.ATC.TTC.TCC.CTC
               ApaI   +++

***: start codon.
+++: termination codon.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCD-F-EIF4B (LMBP 6359) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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