Last data update: 24 January 2024 16:39 CET
Plasmid name: pCD-F-HLH-MALT-C464A-MycHis (LMBP 6101)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6101.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1); mutated sequence HLH dimerization domain; N-terminal FLAG epitope tag; N-terminal His-tag, C-terminal Myc epitope; C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCD-MK; pCD-F-HLH-MALT-C464A |
| Further information: | The plasmid was constructed by isolating the Myc epitope and His-tag as an ApaI/XmaI fragment from the pCD-MK vector and ligated them in frame to the human MALT1 cds in the pCD-F-HLH-MALT-C464A vector. The human MALT1 cds contains a C464A mutation. The plasmid contains a helix-loop-helix domain (HLH) fused to the human MALT1 cds. The HLH domain is a dimerization domain that allows proteins to form homo- and/or heterodimers. The HLH domain consists of the N-terminal sequence of human MALT1 (encoding the first 42 amino acids) and a fragment of the human ets variant gene 6 (ETV6) coding sequence (encoding amino acids 27 to 144). The parental clone of this vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin. The nucleotide sequence of the wild type human MALT1 cds corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2 except for a G instead of T in nucleotide position 4, a C instead of A in position 1393, a C instead of G in position 1395, a T instead of A in position 1488 and a G instead of A in position 1950 of the cds nucleotide sequence. Name mentioned in Malinverni et al. (2010) is F-HLH-MALT-C464A-myc. |
| EMBL Accession number: | AF130356.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 07/10/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: ApaI/XmaI, BamHI, BssHII, HindIII and XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Malinverni et al., Biochem. Biophys. Res. Commun. 400 (2010), 543-547 [PMID: 20804738] [DOI: 10.1016/j.bbrc.2010.08.091] |
| Related plasmid reference: | Elton et al., FEBS J. 283 (2016), 403-412 [PMID: 26573773] [DOI: 10.1111/febs.13597] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCD-F-HLH-MALT-C464A-MycHis (LMBP 6101) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Malinverni et al., 2010. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.