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GeneCorner plasmid details

Last data update: 31 October 2020 04:21 CET

Plasmid name: pCD-F-HLH-MK-bioC (LMBP 5681)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5681.gb (View with Genome Compiler)
p5681.txt
p5681.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1); with Kozak consensus sequence
FLAG epitope tag; N-terminal
HLH dimerization domain; N-terminal
SGSSGSSG linker; C-terminal
Biotinylation sequence (bio-tag); C-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: -
Further information: The helix-loop-helix (HLH) domain is a dimerization domain that allows proteins to form homo- and/or heterodimers.
The HLH domain consists of the N-terminal sequence of hMLT (encoding the first 42 amino acids) and a fragment of the human ets variant gene 6 (hETV6) coding sequence (encoding amino acids 27 to 144).
The bio-tag is biotinylated by cotransfection with the plasmid pMSCV-F-BirA and can be used for highly efficient purification.
The parental clone of this vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726942.1.
The nucleotide sequence of the wt hMLT cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2.
EMBL Accession number: AF130356.2, view at EMBL, GenBank, DDBJ
LT726942.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/11/2007
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: Alw44I/HindIII, ApaI/BamHI, EcoRI/NcoI, NdeI, PdmI/SalI and PstI/XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. Baens(1).
(1) Department of Human Genetics, KULeuven, Belgium
Plasmid reference: -
Related plasmid reference: Noels et al., J. Biol. Chem. 282 (2007), 10180-10189 [PMID: 17287209]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCD-F-HLH-MK-bioC (LMBP 5681) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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