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GeneCorner plasmid details

Last data update: 29 October 2020 04:15 CET

Plasmid name: pCD-F-MALT-p76-deltaC210 (LMBP 7680)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p7680.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1); p76 fragment with C-terminal deletion
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCD-F-MK-p76; pCD-F-MALT1-Ig2-ΔC210
Further information: The plasmid was constructed by cloning the mutated C-terminus of MALT1, originating from pCD-F-MALT1-Ig2-ΔC210, into pCD-F-MK-p76 via the conserved PmlI and ApaI restriction sites.
This eukaryotic expression vector contains the p76 fragment, a C-terminal fragment of MALT1 lacking the N-terminal death domain (starting from AA 150 of full length), missing 210 C-terminal residues additionally.
Other name of the plasmid is pCD-F-MALT-p76-ΔC210.
EMBL Accession number: -
Latest sequence update: 11/06/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Inflammation Research Center, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - VIB/BCCM MTA
- Restricted to academic users
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCD-F-MALT-p76-deltaC210 (LMBP 7680) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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