Last data update: 24 January 2024 16:39 CET
Plasmid name: pCD-F-bio-Card11-L232LI (LMBP 7889)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7889.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse caspase recruitment domain family member 11 cDNA (Card11, BIMP3, CARMA1, GeneID 108723); mutated sequence FLAG epitope tag; N-terminal Biotinylation sequence (bio-tag); N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCD-F |
| Further information: | The mouse Card11 coding sequence contains a L232LI mutation, inserting an isoleucine codon after the leucin codon at position 232. The bio-tag is biotinylated by cotransfection with the plasmid pMSCV-F-BirA and can be used for highly efficient purification. The parental clone of this vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin. The nucleotide sequence of the wild type mouse Card11 coding sequence corresponds with the Genbank accession number NM_175362.2. |
| EMBL Accession number: | NM_175362.2, view at GenBank |
| Latest sequence update: | |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr M. Baens(1). (1) Department of Human Genetics, KULeuven, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Afonina et al., EMBO Rep. 17 (2016), 914-927 [PMID: 27113748] [DOI: 10.15252/embr.201642109] Lenz et al., Science 319 (2008), 1676–1679 [PMID: 18323416] [DOI: 10.1126/science.1153629] Baens et al., PLoS One. 9 (2014), e103774 [PMID: 25105596] [DOI: 10.1371/journal.pone.0103774] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCD-F-bio-Card11-L232LI (LMBP 7889) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.