Last data update: 24 January 2024 16:39 CET
Plasmid name: pCD-F-hCYLD-C (LMBP 6358)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p6358.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human CYLD lysine 63 deubiquitinase cDNA (CYLD, CYLD1, USPL2, GeneID 1540); C-terminal fragment FLAG epitope tag; N-terminal |
Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pCD-F-MALT1; pCD-F-hCYLD |
Further information: | The plasmid was created by PCR amplyfing the C-terminal fragment of hCYLD using pCD-F-hCYLD as a template, and ligating it as an KpnI/ApaI fragment into the KpnI/ApaI opened pCD-F-MALT1 vector. The nucleotide sequence of the wild type human CYLD cDNA corresponds with the EMBL Nucleotide Sequence database accession numberAJ250014.1. Other name of the plasmid is pCD-F-CYLD-C. |
EMBL Accession number: | AJ250014.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 15/09/2011 |
Sequence detail: | Primers used to amplify the C-terminal fragment of hCYLD: Forward: 5' ATGGTACCA.ATG.AAC.AGA.TCT.GAA.TTA.TTT.TAT.ACC KpnI *** Reverse: 5' TAAGGGCCC.TTA.TTT.GTA.CAA.CTC.ATT.GTT.GG ApaI +++ ***: start codon. +++: termination codon. Punctuation indicates reading frame. |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA - Possible extra restrictions defined by the depositor, based on the description of the enduser's research |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCD-F-hCYLD-C (LMBP 6358) is available at BCCM/GeneCorner. This plasmid was deposited by J. Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.