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GeneCorner plasmid details

Last data update: 21 October 2020 04:26 CEST

Plasmid name: pCD-F-mMK (LMBP 5955)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p5955.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse MALT1 paracaspase cDNA (Malt1, Pcasp1)
FLAG epitope tag; N-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pcDNA3.1-FlagC
Further information: The mouse Malt1 cDNA was retrieved from a hybrid mouse (129Sv x C57BL).
The parental clone of this vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin.
The nucleotide sequence of the mouse Malt1 cDNA corresponds with the Genbank accession number NM_172833.2, except for 7 nucleotides in the coding sequence, respectively at position 48 (G-->A), 355 (T-->C), 357 (G-->C), 414 (C-->T), 417 (A-->G), 1071 (A-->G), 1674 (T-->C). However, no amino acid changes are involved.
Name mentioned in Coornaert et al. (2008) is pcDNA3-Flag-mMALT1.
Other name of the plasmid is pcD-F-mMLT.
EMBL Accession number: NM_172833.2, view at GenBank
Latest sequence update: 09/11/2010
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, HindIII, NcoI and PvuII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr M. Baens(1).
(1) Department of Human Genetics, KULeuven, Belgium
Plasmid reference: Coornaert et al., Nat. Immunol. 9 (2008), 263-271 [PMID: 18223652] [DOI: 10.1038/ni1561]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCD-F-mMK (LMBP 5955) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr M. Baens and was published in Coornaert et al., 2008.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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