Last data update: 21 October 2020 04:26 CEST
Plasmid name: pCD-F-mMK (LMBP 5955)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse MALT1 paracaspase cDNA (Malt1, Pcasp1)
FLAG epitope tag; N-terminal
|Promoter:||Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
|Terminator:||Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)
Neomycin (neo; G418)
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Further information:||The mouse Malt1 cDNA was retrieved from a hybrid mouse (129Sv x C57BL).
The parental clone of this vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin.
The nucleotide sequence of the mouse Malt1 cDNA corresponds with the Genbank accession number NM_172833.2, except for 7 nucleotides in the coding sequence, respectively at position 48 (G-->A), 355 (T-->C), 357 (G-->C), 414 (C-->T), 417 (A-->G), 1071 (A-->G), 1674 (T-->C). However, no amino acid changes are involved.
Name mentioned in Coornaert et al. (2008) is pcDNA3-Flag-mMALT1.
Other name of the plasmid is pcD-F-mMLT.
|EMBL Accession number:||NM_172833.2, view at GenBank|
|Latest sequence update:||09/11/2010|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI, HindIII, NcoI and PvuII.|
|History of deposit:||This plasmid was deposited by Prof. Dr M. Baens(1).
(1) Department of Human Genetics, KULeuven, Belgium
|Plasmid reference:||Coornaert et al., Nat. Immunol. 9 (2008), 263-271 [PMID: 18223652] [DOI: 10.1038/ni1561]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCD-F-mMK (LMBP 5955) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr M. Baens and was published in Coornaert et al., 2008.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.