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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pCD-LCK-F (LMBP 5350)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5350.gb (View with Genome Compiler)
p5350.txt
p5350.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse lymphocyte protein tyrosine kinase cDNA (Lck); myristoylation-targeting sequence, N-terminal
FLAG epitope tag; N-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: -
Further information: The plasmid is designed for high-level expression of a recombinant protein, fused to the N-terminal mLck myristoylation-targeting peptide and FLAG epitope tag, under control of the strong hCMV-IE promoter and enhancer in mammalian cells.
The mLck myristoylation-targeting sequence directs the fusion protein to cellular membranes.
The parental clone of this vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727010.1.
Other names of the plasmid are pCD-Lck-Flag-b and pCD-Lck-Flag.
EMBL Accession number: LT727010.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/11/2007
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: Alw44I/BamHI, AvaI/XmnI, HincII, HindIII/PvuII, NaeI/SacI and NdeI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. Baens(1).
(1) Department of Human Genetics, KULeuven, Belgium
Plasmid reference: -
Related plasmid reference: Noels et al., J. Biol. Chem. 282 (2007), 10180-10189 [PMID: 17287209]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCD-LCK-F (LMBP 5350) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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