Last data update: 23 September 2021 14:51 CEST
Plasmid name: pCD-Lck-F-MK (LMBP 5958)
|New search||Print data sheet|
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1)|
Mouse lymphocyte protein tyrosine kinase cDNA (Lck); myristoylation-targeting sequence, N-terminal
FLAG epitope tag; N-terminal
|Promoter:||Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
|Terminator:||Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)
Neomycin (neo; G418)
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Further information:||The plasmid was constructed by cloning the human MALT1 coding sequence into the pCD-Lck-Flag vector.
The mouse Lck myristoylation-targeting sequence directs the fusion protein to cellular membranes.
The parental clone of this vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin.
The nucleotide sequence of the human MALT1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2.
Name mentioned in Baens et al. (2014) is pcD-mp-F-MALT1.
|EMBL Accession number:||AF130356.2, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||13/10/2009|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr M. Baens(1).
(1) Department of Human Genetics, KULeuven, Belgium
|Plasmid reference:||Baens et al., PLoS ONE 9 (2014), e103774 [PMID: 25105596] [DOI: 10.1371/journal.pone.0103774]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCD-Lck-F-MK (LMBP 5958) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens and was published in Baens et al., 2014.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.