Last data update: 24 January 2024 16:39 CET
Plasmid name: pCDM8hFasR (LMBP 3118)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p3118.gb
(View with Genome Compiler) p3118.txt p3118.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human TNF receptor superfamily, member 6 cDNA (FAS) |
Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Suppressor tRNA gene (supF; requires a p3 containing host) |
Replicon: | Bacteriophage M13 origin Escherichia coli plasmid pMB1 origin Polyoma virus origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli; use strains with the p3 helper plasmid (kan resistant, amber codon in amp and tet) Mammalian cells; SV40 permissive cells |
Parental clone: | pCDM8; pBLF58-1 |
Further information: | The plasmid was constructed by ligating the XbaI-XhoI fragment of pBLF58-1, containing the human FAS cDNA, to the XhoI-KpnI and KpnI-XbaI fragments of pCDM8. pCDM8hFasR is a shuttle vector designed for high-level transient expression of human FAS in mammalian cells. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727441.1. The nucleotide sequence of the human FAS cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M67454.1. Other name of the plasmid is pCDM8hFASR. |
EMBL Accession number: | M67454.1, view at EMBL, GenBank, DDBJ LT727441.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 27/11/1995 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BstXI, SpeI and XbaI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by D. Vercammen(1) (2) and Prof. Dr W. Fiers(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | Vercammen et al., Cytokine 9 (1997), 801-808 [PMID: 9367540] |
Related plasmid reference: | Itoh et al., Cell 66 (1991), 233-243 [PMID: 1713127] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061[p3] |
Host reference: | - |
Related host reference: | Instruction Manual (Invitrogen) Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Helper plasmid: | p3 |
Cultivation medium: | LB-Lennox + ampicillin (50 μg/ml) + kanamycin (50 μg/ml) + tetracycline (10 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCDM8hFasR (LMBP 3118) is available at BCCM/GeneCorner. This plasmid was deposited by D. Vercammen and Prof. Dr W. Fiers and was published in Vercammen et al., 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.