Last data update: 24 October 2020 04:12 CEST
Plasmid name: pCDM8hFasR (LMBP 3118)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Human TNF receptor superfamily, member 6 cDNA (FAS)
|Promoter:||Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Suppressor tRNA gene (supF; requires a p3 containing host)|
|Replicon:||Bacteriophage M13 origin
Escherichia coli plasmid pMB1 origin
Polyoma virus origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli; use strains with the p3 helper plasmid (kan resistant, amber codon in amp and tet)
Mammalian cells; SV40 permissive cells
|Parental clone:||pCDM8; pBLF58-1|
|Further information:||The plasmid was constructed by ligating the XbaI-XhoI fragment of pBLF58-1, containing the human FAS cDNA, to the XhoI-KpnI and KpnI-XbaI fragments of pCDM8.
pCDM8hFasR is a shuttle vector designed for high-level transient expression of human FAS in mammalian cells.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727441.1.
The nucleotide sequence of the human FAS cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M67454.1.
Other name of the plasmid is pCDM8hFASR.
|EMBL Accession number:||M67454.1, view at EMBL, GenBank, DDBJ
LT727441.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||27/11/1995|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BstXI, SpeI and XbaI.|
|History of deposit:||This plasmid was deposited by D. Vercammen(1) (2) and Prof. Dr W. Fiers(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Vercammen et al., Cytokine 9 (1997), 801-808 [PMID: 9367540]
|Related plasmid reference:||Itoh et al., Cell 66 (1991), 233-243 [PMID: 1713127]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061[p3]|
|Related host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Invitrogen Instruction Manual
|Cultivation medium:||LB-Lennox + ampicillin (50 μg/ml) + kanamycin (50 μg/ml) + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCDM8hFasR (LMBP 3118) is available at BCCM/GeneCorner. This plasmid was deposited by D. Vercammen and Prof. Dr W. Fiers and was published in Vercammen et al., 1997.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.