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GeneCorner plasmid details

Last data update: 24 October 2020 04:12 CEST

Plasmid name: pCDM8hFasR (LMBP 3118)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p3118.gb (View with Genome Compiler)
p3118.txt
p3118.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human TNF receptor superfamily, member 6 cDNA (FAS)
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Suppressor tRNA gene (supF; requires a p3 containing host)
Replicon: Bacteriophage M13 origin
Escherichia coli plasmid pMB1 origin
Polyoma virus origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli; use strains with the p3 helper plasmid (kan resistant, amber codon in amp and tet)
Mammalian cells; SV40 permissive cells
Parental clone: pCDM8; pBLF58-1
Further information: The plasmid was constructed by ligating the XbaI-XhoI fragment of pBLF58-1, containing the human FAS cDNA, to the XhoI-KpnI and KpnI-XbaI fragments of pCDM8.
pCDM8hFasR is a shuttle vector designed for high-level transient expression of human FAS in mammalian cells.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727441.1.
The nucleotide sequence of the human FAS cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M67454.1.
Other name of the plasmid is pCDM8hFASR.
EMBL Accession number: M67454.1, view at EMBL, GenBank, DDBJ
LT727441.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 27/11/1995
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BstXI, SpeI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by D. Vercammen(1) (2) and Prof. Dr W. Fiers(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Vercammen et al., Cytokine 9 (1997), 801-808 [PMID: 9367540]
Related plasmid reference: Itoh et al., Cell 66 (1991), 233-243 [PMID: 1713127]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061[p3]
Host reference: -
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Invitrogen Instruction Manual
Helper plasmid: p3
Cultivation medium: LB-Lennox + ampicillin (50 μg/ml) + kanamycin (50 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCDM8hFasR (LMBP 3118) is available at BCCM/GeneCorner. This plasmid was deposited by D. Vercammen and Prof. Dr W. Fiers and was published in Vercammen et al., 1997.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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