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GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pCEV4 (LMBP 12055)

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Price category: Cat. 3 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p12055.gb (View with Genome Compiler)
p12055.txt
p12055.pdf
Sequence
analysis results
Genecorner:

NGS: a10-gc-may2021.fasta

Cloned DNA: Nicotiana benthamiana chromosomal integration cassette
Promoter: Nicotiana tabacum ATPase subunit IV promoter (atpI)
Nicotiana tabacum 16S ribosomal RNA promoter (rrn)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Nicotiana tabacum ribosomal protein S16 terminator (rps16)
Nicotiana tabacum photosystem II protein D1 terminator (psbA)
Selection marker: Ampicillin (amp)
Streptomycin (Sm; spectinomycin (Sp))
Replicon: Escherichia coli plasmid pMB1 origin
Chloroplast replication origin (OriA)
Host range: Escherichia coli
Plant cells
Parental clone: pUC19
Further information: The plasmid was constructed by cloning a N. benthamiana chromosomal integration cassette into the pUC19 vector.
The chromosomal integration cassette consists of a cloning site for a gene of interest, under control of the atpI promoter and rps16 terminator, followed by a streptomycin resistance gene (aadA), flanked by N. benthamiana targeting regions for integration via homologous recombination.
The N. benthamiana left targeting region (LTR) contains partial 16S rRNA and full-length trnI and the N. benthamiana right targeting region (RTR) contains full-length trnA and partial 23S rRNA.
The nucleotide sequence of the pCEV4 plasmid corresponds with the EMBL Nucleotide Sequence Database accession number KY930500.1.
EMBL Accession number: KY930500.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 02/12/2019
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, NotI and PvuII.

This plasmid has also been fully sequenced.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K-Y To(1).
(1) Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei, Taiwan
Plasmid reference: Chen et al., in 'Genetic Transformation in Crops, Construction and Evaluation of Chloroplast Expression Vectors in Higher Plants', IntechOpen (2020), 47-75 [ISSN/ISBN: 978-1-83962-451-3] [DOI: 10.5772/intechopen.91887]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5αT1R
Host reference: -
Related host reference: Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCEV4 (LMBP 12055) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K-Y To and was published in Chen et al., 2020.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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