Last data update: 24 January 2024 16:39 CET
Plasmid name: pCEV4 (LMBP 12055)
New search | Print data sheet |
Price category: | Cat. 3 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p12055.gb
(View with Genome Compiler) p12055.txt p12055.pdf |
Sequence analysis results Genecorner: |
NGS: a10-gc-may2021.fasta |
Cloned DNA: |
Nicotiana benthamiana chromosomal integration cassette |
Promoter: | Nicotiana tabacum ATPase subunit IV promoter (atpI) Nicotiana tabacum 16S ribosomal RNA promoter (rrn) Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | Nicotiana tabacum ribosomal protein S16 terminator (rps16) Nicotiana tabacum photosystem II protein D1 terminator (psbA) |
Selection marker: | Ampicillin (amp) Streptomycin (Sm; spectinomycin (Sp)) |
Replicon: | Escherichia coli plasmid pMB1 origin Chloroplast replication origin (OriA) |
Host range: | Escherichia coli Plant cells |
Parental clone: | pUC19 |
Further information: | The plasmid was constructed by cloning a N. benthamiana chromosomal integration cassette into the pUC19 vector. The chromosomal integration cassette consists of a cloning site for a gene of interest, under control of the atpI promoter and rps16 terminator, followed by a streptomycin resistance gene (aadA), flanked by N. benthamiana targeting regions for integration via homologous recombination. The N. benthamiana left targeting region (LTR) contains partial 16S rRNA and full-length trnI and the N. benthamiana right targeting region (RTR) contains full-length trnA and partial 23S rRNA. The nucleotide sequence of the pCEV4 plasmid corresponds with the EMBL Nucleotide Sequence Database accession number KY930500.1. |
EMBL Accession number: | KY930500.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 02/12/2019 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, NotI and PvuII. This plasmid has also been fully sequenced. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr K-Y To(1). (1) Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei, Taiwan |
Plasmid reference: | Chen et al., in 'Genetic Transformation in Crops, Construction and Evaluation of Chloroplast Expression Vectors in Higher Plants', IntechOpen (2020), 47-75 [ISSN/ISBN: 978-1-83962-451-3] [DOI: 10.5772/intechopen.91887] |
Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5αT1R |
Host reference: | - |
Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCEV4 (LMBP 12055) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K-Y To and was published in Chen et al., 2020. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.