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GeneCorner plasmid details

Last data update: 21 October 2021 10:13 CEST

Plasmid name: pCR3-EGFPC-GW (LMBP 8375)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p8375.gb (View with Genome Compiler)
p8375.txt
p8375.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP), C-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Escherichia coli lac operon promoter; mutant (lacUV5)
Simian virus 40 early promoter (SV40 early)
Phage T7 gene 10 promoter (T7g10)
Phage SP6 promoter
Ribosome
binding site:
-
Terminator: Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Bovine growth hormone polyadenylation signal (BGH polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli; use a ccdB-resistant strain for propagation
Mammalian cells; SV40 permissive cells
Parental clone: pCR3
Further information: This is a Gateway destination vector meant for C-terminal fusion of the EGFP reporter gene.
This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726802.1.
The EGFP insert has been sequenced by GeneCorner.
The nucleotide sequence of EGFP corresponds with the EMBL Nucleotide Sequence Database accession number U55761.1.
EMBL Accession number: U55761.1, view at EMBL, GenBank, DDBJ
LT726802.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 27/10/2014
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HindIII/XbaI, NcoI, PstI and XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Goethals(1) and Prof. Dr V. Timmerman(1). It was constructed by Dr L. de Almeida Souza(2).
(1) Department of Molecular Genetics, VIB, Antwerp, Belgium
(2) Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, UK
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB DB3.1
Host reference: -
Related host reference: Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin.
Other culture collection numbers: -

Refer in your Materials and Methods:

pCR3-EGFPC-GW (LMBP 8375) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Goethals and Prof. Dr V. Timmerman.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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