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GeneCorner plasmid details

Last data update: 23 September 2021 14:51 CEST

Plasmid name: pCSN43 (LMBP 8047)

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Price category: Cat. 3 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p8047.gb (View with Genome Compiler)
p8047.txt
p8047.pdf
Sequence
analysis results
Genecorner:

NGS: d05-gc-dec2018.fasta

Cloned DNA: -
Promoter: Aspergillus nidulans tryptophan C promoter (trpC)
Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: Aspergillus nidulans tryptophan C terminator (trpC)
Selection marker: Ampicillin (amp)
Hygromycin (hyg)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Neurospora sp.
Ascomycete fungi
Parental clone: pDH25; pBluescriptIISK+
Further information: The plasmid was constructed by cloning the SalI fragment from pDH25, containing the hygromycin resistance gene under control of the A. nidulans trpC promoter and terminator, into the SalI-opened pBluescriptIISK+ vector. The orientation of the hygromycin cassette is opposite compared to pCSN44.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726869.1.
The nucleotide sequences of the trpC promoter and terminator correspond with the EMBL Nucleotide Sequence Database accession number X02390.1.
EMBL Accession number: X02390.1, view at EMBL, GenBank, DDBJ
LT726869.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 14/01/2013
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRV, PvuI and SalI.

This plasmid has also been fully sequenced but the NGS sequence data still need to be implemented.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr K. McCluskey(1).
(1) FGSC, University of Missouri, Kansas City, USA
Plasmid reference: Staben et al., Fungal Genet. Newsl. 36 (1989), 79-81
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCSN43 (LMBP 8047) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Staben et al., 1989.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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