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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: pCaMET15 (LMBP 4218)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4218.gb (View with Genome Compiler)
p4218.txt
p4218.pdf
Sequence
analysis results
Genecorner:

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Cloned DNA: Candida albicans bifunctional cysteine synthase/O-acetylhomoserine aminocarboxypropyltransferase genomic DNA (MET15, GeneID 3645031)
Promoter: Candida albicans bifunctional cysteine synthase/O-acetylhomoserine aminocarboxypropyltransferase promoter (MET15)
Escherichia coli lac operon promoter
Phage SP6 promoter
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Candida albicans O-acetylhomoserine O-acetylserine sulfhydrylase terminator (CaMET15)
Selection marker: Ampicillin (amp)
Candida albicans URA3; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Candida albicans; ura3(-), integrative
Parental clone: pGAL1PNiST-1; YCpCaMET15
Further information: The plasmid was constructed by inserting C. albicans MET15 genomic DNA as a Sau3AI/EcoRI fragment of YCpCaMET15 between the BglII and BamHI sites of pGAL1PNiST-1. All restriction sites were blunted with T4 DNA polymerase. The orientation of the inserted fragment is antisense relative to the URA3 gene and opposite as to the schematic representation in Viaene et al. (2000).
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727328.1.
The nucleotide sequence of the CaMET15 genomic DNA corresponds with the EMBL Nucleotide Sequence Database accession number AF188275.1.
The nucleotide sequence of the CaURA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198.1. HOWEVER, THERE MIGHT BE CORRESPONDENCE WITH AF109400.1: see LMBP 4100.
EMBL Accession number: X14198.1, view at EMBL, GenBank, DDBJ
AF188275.1, view at EMBL, GenBank, DDBJ
LT727328.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 09/08/2001
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BanII, BstEII, HindII and ScaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Janssen Pharmaceutica N.V.(1). It was constructed by P. Tiels(2) (3).
(1) Beerse, Belgium
(2) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(3) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Viaene et al., Yeast 16 (2000), 1205-1215 [PMID: 10992284]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCaMET15 (LMBP 4218) is available at BCCM/GeneCorner. This plasmid was deposited by Janssen Pharmaceutica N.V. and was published in Viaene et al., 2000.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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