Last data update: 24 January 2024 16:39 CET
Plasmid name: pCal-c-AK (LMBP 5463)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p5463.gb
(View with Genome Compiler) p5463.txt p5463.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human apoptosis inhibitor 2 cDNA (API2, BIRC3); with Kozak consensus sequence Thrombin recognition site; C-terminal Calmodulin-binding peptide tag (CBP); C-terminal Escherichia coli lac repressor gene (lacI) |
Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) Escherichia coli lac repressor promoter (lacI) |
Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
Terminator: | Phage T7 gene 10 terminator (T7g10) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pCAL-c |
Further information: | The plasmid contains the hAPI2 coding sequence, fused to the C-terminal thrombin recognition site and calmodulin-binding peptide tag. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726906.1. The nucleotide sequence of the wt hAPI2 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number BC037420.1. The nucleotide sequence of the pCAL-c vector corresponds with the EMBL Nucleotide Sequence Database accession number U36452.1. |
EMBL Accession number: | BC037420.1, view at EMBL, GenBank, DDBJ U36452.1, view at EMBL, GenBank, DDBJ LT726906.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 28/11/2007 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AatII/Alw44I, BamHI/PdmI, BglII/PstI, HincII/PaeI, KpnI and NcoI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr M. Baens(1). (1) Department of Human Genetics, KULeuven, Belgium |
Plasmid reference: | - |
Related plasmid reference: | Noels et al., J. Biol. Chem. 282 (2007), 10180-10189 [PMID: 17287209] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCal-c-AK (LMBP 5463) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.