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GeneCorner plasmid details

Last data update: 29 November 2020 04:28 CET

Plasmid name: pCal-n-A7M8K (LMBP 5465)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5465.gb (View with Genome Compiler)
p5465.txt
p5465.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human apoptosis inhibitor 2 cDNA (API2, BIRC3); N-terminal fragment (exon 1 --> 7) with Kozak consensus sequence
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1); C-terminal fragment (exon 8 --> end)
Thrombin recognition site; N-terminal
Calmodulin-binding peptide tag (CBP); N-terminal
Escherichia coli lac repressor gene (lacI)
Promoter: Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac)
Escherichia coli lac repressor promoter (lacI)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage T7 gene 10 terminator (T7g10)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pCAL-n
Further information: The plasmid contains the N-terminal fragment of the hAPI2 coding sequence (exon 1 --> exon 7), fused to the C-terminal fragment of the hMLT coding sequence (exon 8 --> end) and to the N-terminal calmodulin-binding peptide tag and thrombin recognition site.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726908.1.
The nucleotide sequence of the wt hAPI2 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number BC037420.1.
The nucleotide sequence of the wt hMLT cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2.
The nucleotide sequence of the pCAL-n vector corresponds with the EMBL Nucleotide Sequence Database accession number U36454.1.
EMBL Accession number: BC037420.1, view at EMBL, GenBank, DDBJ
AF130356.2, view at EMBL, GenBank, DDBJ
U36454.1, view at EMBL, GenBank, DDBJ
LT726908.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/11/2007
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AatII/Alw44I, BamHI/PdmI, BglII/PstI, HincII/PaeI, HindIII and NcoI/SacI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. Baens(1).
(1) Department of Human Genetics, KULeuven, Belgium
Plasmid reference: -
Related plasmid reference: Noels et al., J. Biol. Chem. 282 (2007), 10180-10189 [PMID: 17287209]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCal-n-A7M8K (LMBP 5465) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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