Last data update: 24 January 2024 16:39 CET
Plasmid name: pCal-n-A7M8K (LMBP 5465)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p5465.gb
(View with Genome Compiler) p5465.txt p5465.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human apoptosis inhibitor 2 cDNA (API2, BIRC3); N-terminal fragment (exon 1 --> 7) with Kozak consensus sequence Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1); C-terminal fragment (exon 8 --> end) Thrombin recognition site; N-terminal Calmodulin-binding peptide tag (CBP); N-terminal Escherichia coli lac repressor gene (lacI) |
Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) Escherichia coli lac repressor promoter (lacI) |
Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
Terminator: | Phage T7 gene 10 terminator (T7g10) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pCAL-n |
Further information: | The plasmid contains the N-terminal fragment of the hAPI2 coding sequence (exon 1 --> exon 7), fused to the C-terminal fragment of the hMLT coding sequence (exon 8 --> end) and to the N-terminal calmodulin-binding peptide tag and thrombin recognition site. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726908.1. The nucleotide sequence of the wt hAPI2 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number BC037420.1. The nucleotide sequence of the wt hMLT cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2. The nucleotide sequence of the pCAL-n vector corresponds with the EMBL Nucleotide Sequence Database accession number U36454.1. |
EMBL Accession number: | BC037420.1, view at EMBL, GenBank, DDBJ AF130356.2, view at EMBL, GenBank, DDBJ U36454.1, view at EMBL, GenBank, DDBJ LT726908.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 28/11/2007 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AatII/Alw44I, BamHI/PdmI, BglII/PstI, HincII/PaeI, HindIII and NcoI/SacI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr M. Baens(1). (1) Department of Human Genetics, KULeuven, Belgium |
Plasmid reference: | - |
Related plasmid reference: | Noels et al., J. Biol. Chem. 282 (2007), 10180-10189 [PMID: 17287209] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCal-n-A7M8K (LMBP 5465) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.