Last data update: 21 October 2021 10:13 CEST
Plasmid name: pCi-C-TagBFP-DEST (LMBP 9838)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)|
Entacmaea quadricolor red fluorescent protein DNA (eqFP578, RFP); bright monomeric blue fluorescent variant (TagBFP), C-terminal
|Promoter:||Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Escherichia coli lac operon promoter; mutant (lacUV5)
Phage T7 gene 10 promoter (T7g10)
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli; use a ccdB-resistant strain for propagation
Mammalian cells; SV40 permissive cells
|Further information:||The plasmid was created by cloning the Gateway recombination cassette and the TagBFP coding sequence into the pCI vector.
TagBFP is a monomeric blue fluorescent protein generated by site-specific and random mutagenesis of TagRFP. TagBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.
This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner.
Other name of the plasmid is pCi C-BFP2 DEST.
|EMBL Accession number:||-|
|Latest sequence update:||09/05/2016|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||The plasmid was deposited by Prof. Dr H. McMahon(1). It was constructed by Dr S. Lucken-Ardjomande Hasler(1).
(1) Medical Research Council - Laboratory of Molecular Biology, Neurobiology Division, Cambridge University, Cambridge, United Kingdom
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12xB DB3.1|
|Related host reference:||Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)*|
|Cultivation remark:||*: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin.|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCi-C-TagBFP-DEST (LMBP 9838) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr H. McMahon.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.