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GeneCorner plasmid details

Last data update: 21 October 2021 10:13 CEST

Plasmid name: pCi-C-TagBFP-DEST (LMBP 9838)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9838.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Entacmaea quadricolor red fluorescent protein DNA (eqFP578, RFP); bright monomeric blue fluorescent variant (TagBFP), C-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Escherichia coli lac operon promoter; mutant (lacUV5)
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli; use a ccdB-resistant strain for propagation
Mammalian cells; SV40 permissive cells
Parental clone: pCI
Further information: The plasmid was created by cloning the Gateway recombination cassette and the TagBFP coding sequence into the pCI vector.
TagBFP is a monomeric blue fluorescent protein generated by site-specific and random mutagenesis of TagRFP. TagBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.
This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner.
Other name of the plasmid is pCi C-BFP2 DEST.
EMBL Accession number: -
Latest sequence update: 09/05/2016
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Prof. Dr H. McMahon(1). It was constructed by Dr S. Lucken-Ardjomande Hasler(1).
(1) Medical Research Council - Laboratory of Molecular Biology, Neurobiology Division, Cambridge University, Cambridge, United Kingdom
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB DB3.1
Host reference: -
Related host reference: Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin.
Other culture collection numbers: -

Refer in your Materials and Methods:

pCi-C-TagBFP-DEST (LMBP 9838) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr H. McMahon.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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