Last data update: 12 August 2022 09:30 CEST
Plasmid name: pCi-C-myc-DEST (LMBP 9840)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9840.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene) Myc epitope; C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli lac operon promoter; mutant (lacUV5) Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli; use a ccdB-resistant strain for propagation Mammalian cells |
| Parental clone: | pCI |
| Further information: | The plasmid was created by cloning the Gateway recombination cassette and the myc epitope into the pCI vector. This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner. Other name of the plasmid is pCi C-myc DEST. |
| EMBL Accession number: | - |
| Latest sequence update: | 09/05/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr H. McMahon(1). It was constructed by Dr S. Lucken-Ardjomande Hasler(1). (1) Medical Research Council - Laboratory of Molecular Biology, Neurobiology Division, Cambridge University, Cambridge, United Kingdom |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB DB3.1 |
| Host reference: | - |
| Related host reference: | Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCi-C-myc-DEST (LMBP 9840) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr H. McMahon. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.