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GeneCorner plasmid details

Last data update: 21 October 2021 10:13 CEST

Plasmid name: pCi-N-EGFP-DEST (LMBP 9843)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p9843.gb (View with Genome Compiler)
p9843.txt
p9843.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP), N-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Escherichia coli lac operon promoter; mutant (lacUV5)
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli; use a ccdB-resistant strain for propagation
Mammalian cells
Parental clone: pCI
Further information: The plasmid was created by cloning the EGFP coding sequence and the Gateway recombination cassette into the pCI vector.
This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner.
Other name of the plasmid is pCi N-EGFP DEST.
EMBL Accession number: -
Latest sequence update: 09/05/2016
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, HindIII, HpaI/NheI and NcoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Prof. Dr H. McMahon(1). It was constructed by Dr S. Lucken-Ardjomande Hasler(1).
(1) Medical Research Council - Laboratory of Molecular Biology, Neurobiology Division, Cambridge University, Cambridge, United Kingdom
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB DB3.1
Host reference: -
Related host reference: Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin.
Other culture collection numbers: -

Refer in your Materials and Methods:

pCi-N-EGFP-DEST (LMBP 9843) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr H. McMahon.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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