GREAT AT SMALL THINGS

0

GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pD5-gpt-GPx (LMBP 3479)

Add to cart

New search Print data sheet
Price category: Cat. 2 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: not available
Sequence
analysis results
Genecorner:

NGS: a01-gc-dec2019-partial.fasta

Cloned DNA: Bovine selenium glutathione peroxidase cDNA (bGPx)
Promoter: Adenovirus 5 late promoter (Ad5 late)
Simian virus 40 promoter (SV40)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Xanthine-guanine phosphoribosyltransferase (XGPRT)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Mammalian cells
Parental clone: pSPT19-GPx; pD5-gpt
Further information: The plasmid was constructed as follows: the EcoRI fragment from pST19-GPx, containing the bovine selenium glutathione peroxidase cDNA (bGPx), was blunted, provided with BamHI linkers and inserted into the unique BamHI site of pD5-gpt.
Other name of the plasmid is pD5-gpt/GPx bovine clone 5 sense.
EMBL Accession number: -
Latest sequence update: 17/05/2001
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI.

This plasmid has also been fully sequenced but the NGS sequence data were incomplete and they also still need to be implemented.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Amstad(1).
(1) University of Maryland, USA
Plasmid reference: Amstad et al., J. Biol. Chem. 269 (1994), 1606-1609 [PMID: 8294405]
Related plasmid reference: Amstad et al., Biochemistry 30 (1991), 9305-9313 [PMID: 1654093]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB HB101
Host reference: Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
Related host reference: Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8]
Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pD5-gpt-GPx (LMBP 3479) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Amstad and was published in Amstad et al., 1994.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search