Last data update: 24 January 2024 16:39 CET
Plasmid name: pD5-gpt-GPx (LMBP 3479)
New search | Print data sheet |
Price category: | Cat. 2 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: | not available |
Sequence analysis results Genecorner: |
|
Cloned DNA: |
Bovine selenium glutathione peroxidase cDNA (bGPx) |
Promoter: | Adenovirus 5 late promoter (Ad5 late) Simian virus 40 promoter (SV40) |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) Xanthine-guanine phosphoribosyltransferase (XGPRT) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli Mammalian cells |
Parental clone: | pSPT19-GPx; pD5-gpt |
Further information: | The plasmid was constructed as follows: the EcoRI fragment from pST19-GPx, containing the bovine selenium glutathione peroxidase cDNA (bGPx), was blunted, provided with BamHI linkers and inserted into the unique BamHI site of pD5-gpt. Other name of the plasmid is pD5-gpt/GPx bovine clone 5 sense. |
EMBL Accession number: | - |
Latest sequence update: | 17/05/2001 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI. This plasmid has also been fully sequenced but the NGS sequence data were incomplete and they also still need to be implemented. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr P. Amstad(1). (1) University of Maryland, USA |
Plasmid reference: | Amstad et al., J. Biol. Chem. 269 (1994), 1606-1609 [PMID: 8294405] |
Related plasmid reference: | Amstad et al., Biochemistry 30 (1991), 9305-9313 [PMID: 1654093] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12xB HB101 |
Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pD5-gpt-GPx (LMBP 3479) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Amstad and was published in Amstad et al., 1994. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.