GREAT AT SMALL THINGS

0

GeneCorner plasmid details

Last data update: 21 October 2021 10:13 CEST

Plasmid name: pDEST-myr (LMBP 9234)

Add to cart

New search Print data sheet
Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9234.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Mouse lymphocyte protein tyrosine kinase cDNA (Lck); myristoylation-targeting sequence, N-terminal
FLAG epitope tag; N-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage T7 gene 10 promoter (T7g10)
Simian virus 40 early promoter (SV40 early)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli; use a ccdB-resistant strain for propagation
Mammalian cells; SV40 permissive cells
Parental clone: pCD-Lck-Flag; pdcDNA-FLAG
Further information: The Gateway destination cassette was PCR-amplified from pdcDNA-FLAG and inserted into the XhoI/ApaI-opened pCD-Lck-Flag plasmid. This results in a Gateway destination vector where the expressed protein is flag tagged and contains a myristoylation tag, both at the N-terminus.
This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner.
EMBL Accession number: -
Latest sequence update: 19/06/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. De Ceuleneer(1) and Prof. Dr R. Beyaert(1).
(1) BCCM/GeneCorner, Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB DB3.1
Host reference: -
Related host reference: Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pDEST-myr (LMBP 9234) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. De Ceuleneer and Prof. Dr R. Beyaert.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search