Last data update: 24 January 2024 16:39 CET
Plasmid name: pDsRed-N-GW (LMBP 8365)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p8365.gb
(View with Genome Compiler) p8365.txt p8365.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene) Discosoma sp. red fluorescent protein DNA (DsRed1); synthetic monomeric variant (mDsRed), N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli lac operon promoter; mutant (lacUV5) Escherichia coli β-lactamase promoter (amp) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli; use a ccdB-resistant strain for propagation Mammalian cells; SV40 permissive cells |
| Parental clone: | pDsRed-C1 |
| Further information: | This is a Gateway destination vector meant for N-terminal fusion of the DsRed reporter gene. This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726797.1. The nucleotide sequence of mDsRed corresponds with the EMBL Nucleotide Sequence Database accession number EU827527.1. |
| EMBL Accession number: | EU827527.1, view at EMBL, GenBank, DDBJ LT726797.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/06/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HpaI/NheI, NcoI and NotI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr S. Goethals(1) and Prof. Dr V. Timmerman(1). It was constructed by Dr L. de Almeida Souza(2). (1) Department of Molecular Genetics, VIB, Antwerp, Belgium (2) Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, UK |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB DB3.1 |
| Host reference: | - |
| Related host reference: | Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324] |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with kanamycin. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pDsRed-N-GW (LMBP 8365) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Goethals and Prof. Dr V. Timmerman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.