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GeneCorner plasmid details

Last data update: 23 September 2021 14:51 CEST

Plasmid name: pEF-BOS (LMBP 4456)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4456.gb (View with Genome Compiler)
p4456.txt
p4456.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Human elongation factor 1α promoter (EF1α)
Escherichia coli lac operon promoter
Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Human granulocyte macrophage colony stimulating factor polyadenylation signal (GM-CSF polyA)
Selection marker: Ampicillin (amp)
Replicon: Bacteriophage M13 origin
Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pUC19; pCDM8
Further information: This plasmid gives powerful mammalian expression, also for antisense RNA; inserts are to be cloned by exchanging the stuffer fragment between the XbaI or BstXI sites.
Use L929, HeLa, Ch4-2 or COS cells for expression.
The BstXI restriction site, present in the human elongation factor 1α promoter, has been removed.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727211.1.
The nucleotide sequence of this plasmid, originally provided by Dr S. Nagata, has been adjusted to the pCDM8 sequence update.
EMBL Accession number: LT727211.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/11/1995
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglI, BglII, EcoRI, HindIII/PvuI, NaeI, NarI/XmnI, NcoI, NotI, PstI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Nagata(1).
(1) University Medical School, Osaka, Japan
Plasmid reference: Mizushima et al., Nucleic Acids Res. 18 (1990), 5322 [PMID: 1698283]
Related plasmid reference: Liberal et al., Cell Cycle 9 (2010), 4222-4227 [PMID: 20962587] [DOI: 10.4161/cc.9.20.13544]
Uetsuki et al., J. Biol. Chem. 264 (1989), 5791-5798 [PMID: 2564392]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pEF-BOS (LMBP 4456) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Nagata and was published in Mizushima et al., 1990.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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