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GeneCorner plasmid details

Last data update: 12 May 2021 19:50 CEST

Plasmid name: pENTR1A-NLS-LacZ (LMBP 8208)

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Price category: Cat. 3 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p8208.gb (View with Genome Compiler)
p8208.txt
p8208.pdf
Sequence
analysis results
Genecorner:

Sanger: 104828.fasta

Sanger: 104829.fasta

NGS: d12-gc-dec2018.fasta

Cloned DNA: Escherichia coli lac Z gene (lacZ); with modified 5' end
SV40 large T-antigen nuclear localization signal (NLS); N-terminal
Promoter: -
Ribosome
binding site:
-
Terminator: Escherichia coli rrnB operon T1 terminator
Escherichia coli rrnB operon T2 terminator
Selection marker: Neomycin (neo; kanamycin (kan))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pENTR1A
Further information: This Gateway entry vector contains the lacZ coding sequence flanked by attL1-L2 sites.
The plasmid can be used as a positive middle entry control vector in the construction of Gateway-compatible Rosa26 locus targeting expression vectors.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726876.1.
The region upstream from the Tn903 neomycin resistance, including the 5' end of lacZ, and the region downstream from the pMB1 ori, including the 3' end of lacZ, were sequenced by BCCM/GeneCorner.
Other name of the plasmid is pENTR1A nls-LacZ.
EMBL Accession number: LT726876.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 22/11/2013
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: DraI, HincII/NsiI, NcoI, NdeI and PvuI.

This plasmid has also been fully sequenced but the NGS sequence data still need to be implemented.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr L. Haenebalcke(1) (2) and Prof. Dr Jody Haigh(3).
(1) VIB Inflammation Research Center, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
(3) Division of Blood Cancers, Australian Centre for Blood Diseases (ACBD), Monash University & Alfred Health, Melbourne, Australia
Plasmid reference: -
Related plasmid reference: PhD thesis Lieven Haenebalcke
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pENTR1A-NLS-LacZ (LMBP 8208) is available at BCCM/GeneCorner. This plasmid was deposited by Dr L. Haenebalcke and Prof. Dr Jody Haigh.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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