Last data update: 26 November 2020 04:30 CET
Plasmid name: pENTR1A-OSKM (LMBP 8210)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Thosea asigna virus 2A peptide (T2A)
Foot-and-mouth disease virus 2A peptide (F2A)
Mouse Kruppel-like factor 4 (gut) cDNA (Klf4, EZF, Gklf, Zie)
Mouse myelocytomatosis oncogene cDNA (Myc, bHLHe39, c-myc, Myc2, Niard, Nird)
Mouse SRY (sex determining region Y)-box 2 cDNA (Sox2, Icc, Sox-2, ysb)
Mouse POU domain, class 5, transcription factor 1 cDNA (Pou5f1, Oct-3, Oct-3/4, Oct3/4, Oct4, Oct-4, Otf3, Otf-3, Otf3g, Otf3-rs7, Otf4, Otf-4)
|Terminator:||Escherichia coli rrnB operon T1 terminator
Escherichia coli rrnB operon T2 terminator
|Selection marker:||Neomycin (neo; kanamycin (kan))|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||This Gateway Entry vector was constructed by cloning the mouse OSKM cassette into the pENTR1A vector.
The coding sequences in the OSKM cassette are connected via the self-cleaving T2A and F2A peptides, fulfilling the same role as an IRES, but with a much shorter sequence.
Other name of the plasmid is pENTR1A OSKM.
|EMBL Accession number:||-|
|Latest sequence update:||01/12/2016|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr J. Haigh(1).
(1) Australian Centre for Blood Diseases, Central Clinical School, Monash University, Melbourne, Australia
|Plasmid reference:||PhD thesis Lieven Haenebalcke
|Related plasmid reference:||Yusa et al., Nat. Methods 6 (2009), 363-369 [PMID: 19337237]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
|Cultivation medium:||LB-Lennox + kanamycin (50 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pENTR1A-OSKM (LMBP 8210) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J. Haigh and was published in Lieven Haenebalcke, .|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.