Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR1A-OSKM (LMBP 8210)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8210.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Thosea asigna virus 2A peptide (T2A) Foot-and-mouth disease virus 2A peptide (F2A) Mouse Kruppel-like factor 4 (gut) cDNA (Klf4, EZF, Gklf, Zie) Mouse myelocytomatosis oncogene cDNA (Myc, bHLHe39, c-myc, Myc2, Niard, Nird) Mouse SRY (sex determining region Y)-box 2 cDNA (Sox2, Icc, Sox-2, ysb) Mouse POU domain, class 5, transcription factor 1 cDNA (Pou5f1, Oct-3, Oct-3/4, Oct3/4, Oct4, Oct-4, Otf3, Otf-3, Otf3g, Otf3-rs7, Otf4, Otf-4) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pENTR1A |
| Further information: | This Gateway Entry vector was constructed by cloning the mouse OSKM cassette into the pENTR1A vector. The coding sequences in the OSKM cassette are connected via the self-cleaving T2A and F2A peptides, fulfilling the same role as an IRES, but with a much shorter sequence. Other name of the plasmid is pENTR1A OSKM. |
| EMBL Accession number: | - |
| Latest sequence update: | 01/12/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr J. Haigh(1). (1) Australian Centre for Blood Diseases, Central Clinical School, Monash University, Melbourne, Australia |
| Plasmid reference: | PhD thesis Lieven Haenebalcke |
| Related plasmid reference: | Yusa et al., Nat. Methods 6 (2009), 363-369 [PMID: 19337237] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR1A-OSKM (LMBP 8210) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J. Haigh and was published in Lieven Haenebalcke, . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.