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GeneCorner plasmid details

Last data update: 17 September 2021 10:55 CEST

Plasmid name: pET3E-His-AU1-hUb (LMBP 5915)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p5915.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human ubiquitin B cDNA (UBB, Ub, HEL-S-50, GeneID 7314)
Histidine tag (His-tag); N-terminal
AU1-tag; N-terminal
Promoter: Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage T7 gene 10 terminator (T7g10)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pET3E
Further information: This plasmid contains the T7 gene 10 promoter whose transcription is initiated by T7 RNA polymerase, making the vector useful for in vitro preparation of single-stranded RNAs from cloned human UB gene.
EMBL Accession number: BT020104, view at EMBL, GenBank, DDBJ
Latest sequence update: 06/03/2009
Sequence detail:
Schematic representation of the human ubiquitin:
 
                 6  11 27 29 33 48 63
wild type:  5' --K--K--K--K--K--K--K-- 3'
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr K.-L. Guan(1). It was received from Dr. E. Tang(1).
(1) Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA
Plasmid reference: -
Related plasmid reference: Tang et al., J. Biol. Chem. 278 (2003), 37297-37305 [PMID: 12867425]
Restricted distribution: - BCCM/MTA adapted by Michigan University
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pET3E-His-AU1-hUb (LMBP 5915) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K.-L. Guan.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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