Last data update: 24 January 2024 16:39 CET
Plasmid name: pET3E-His-AU1-hUb (LMBP 5915)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p5915.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human ubiquitin B cDNA (UBB, Ub, HEL-S-50, GeneID 7314) Histidine tag (His-tag); N-terminal AU1-tag; N-terminal |
Promoter: | Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
Terminator: | Phage T7 gene 10 terminator (T7g10) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pET3E |
Further information: | This plasmid contains the T7 gene 10 promoter whose transcription is initiated by T7 RNA polymerase, making the vector useful for in vitro preparation of single-stranded RNAs from cloned human UB gene. |
EMBL Accession number: | BT020104, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 06/03/2009 |
Sequence detail: | Schematic representation of the human ubiquitin: 6 11 27 29 33 48 63 wild type: 5' --K--K--K--K--K--K--K-- 3' |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr K.-L. Guan(1). It was received from Dr. E. Tang(1). (1) Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA |
Plasmid reference: | - |
Related plasmid reference: | Tang et al., J. Biol. Chem. 278 (2003), 37297-37305 [PMID: 12867425] |
Restricted distribution: | - BCCM/MTA adapted by Michigan University |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET3E-His-AU1-hUb (LMBP 5915) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K.-L. Guan. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.